Forsdyke Laboratory of Bioinformatics and Theoretical Biology
Keywords: Bioinformatics, Genomics, Theoretical Biology, Speciation, AIDS, Evolution, Biohistory, Darwin, Butler, Romanes, Bateson, Goldschmidt, Chargaff, Wada, G0/G1 switch genes (G0S genes), Immunology, Malaria
Department of Biomedical and Molecular Sciences, Botterell Hall, Queen's University, Kingston, Ontario, Canada, K7L3N6.
- Donald Forsdyke
- Recent Collaborators:
- Dr. Anthony D. Cristillo Grad. Stud. (1993-97) to NIH (1998-02). Advanced BioScience Laboratories, Maryland, USA (2002- )
- Dr. Scott P. Heximer Grad. Stud. (1990-96) to St. Louis, Mo (1997-02). Assist. Prof. at Dept. Physiol., Univ. Toronto (2003- )
- LaVerne Russell Technical assistant (1979-1997) to well earned domesticity (garden, flute, travel and canine care)
- Sheldon Bell 1996. Undergraduate student. To software design for Royal Bank of Canada.
- Labonnie Biswas 1995. Undergraduate student. To Graduate Studies, U. of Toronto.
- Kha Dang 1997. Undergraduate student. To further education
- Previn Dutt 1997. Undergraduate student. To Graduate Studies in Biochemistry, Queen's.
- Tim Lillicrap 1997 High School student, the University of Toronto and then Graduate Studies at Queen's and Oxford.
- Janet Ho 1997. Undergraduate student. Graduate Studies in Biochemistry, Queen's.
- Y.C.(Derek) Chow 1997. Undergraduate student. To Dental School.
- Isabelle Barrette 1998. Undergrad. student. M.Sc. Bioinformatics U. Montreal, Ph.D. U. Alberta, Assist. Prof. Biology, U. Calgary (2006- )
Sean McKenna 1998 Undergraduate student. Graduate studies at U. of Alberta. Assos. Prof. at Dept. Chemistry, U. Manitoba
David. R. Taylor 1998 Undergraduate student. To Medical School
- Perry J. Lao 1999. Undergraduate student. Ottawa Law School graduated 2002. Deceased 2006 after 10K run.
Gregory Hill 1999. Undergraduate student. To start own software company.
Robert Rasile 1999. Undergraduate student. To further education.
James R. Mortimer 2000. Undergraduate student. To bioinformatics with Merck-Frosst.
- Theresa M. St. Denis 2000. Undergraduate student. To MJS BioLynx Inc.
- Alexander K. Schramm 2000. Undergraduate student. To medical school
- Robin Lambros 2000. Undergraduate student. To biotech. industry
- Chris Madill 2000-1. Undergraduate student. To Molecular Mining Inc., Kingston and Grad. studies at Hosp. for Sick Children, Toronto
- Scott. D. Smith 2001. Undergraduate student. To Grad. studies as Hosp. for Sick Children, Toronto
H. Y. (Joanne) Xue 2002. Undergraduate student. To Grad studies at University of Toronto.
S-J. (Jessica) Lee 2002. Undergraduate student. To Grad studies at U BC and then McGill.
- Justin LeBlanc 2003. Undergraduate student. To Grad studies at U BC.
- Jonathan Rayment 2004. Undergraduate student. To Grad studies at McGill University and on to medical school.
- Feng-Hsu (Nelson) Lin 2005. Undergraduate student. To Grad studies at Queen's University.
- Virgil R. Reese 2007- . Independent scholar. Bartlesville, Oklahoma.
- Dr. Chiyu Zhang 2008.Jiangsu University School of Medical Technology, Zhenjiang; 2012 to Institut Pasteur of Shanghai, Chinese Academy of Sciences.
- Dr. Ji-Fu Wei 2008. The Clinical Experiment Center, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu.
- Projects (Bioinformatics/Genomics/Theoretical Biology)
- Self/Not-self discrimination (extracellular and intracellular)
- Speciation, Haldane's Rule
- X-chromosome dosage compensation
- Evolution of genetic dominance
- Folding of randomized-sequence difference analysis
- Chargaff difference analysis: purine-loading of RNAs
- Role of CHI sequences in recombination
- Origin of Introns, "Junk" DNA and low complexity elements
- Isochores (micro and macro)
- Conflict between different forms of information in selected genes/genomes:
- G0S genes
- MHC genes
- Retroviruses (HIV, HTLV)
- Herpes simplex virus
- Epstein-Barr virus
- Plasmodium falciparum
- Saccharomyces cerevisiae (chromosome III)
- Bithorax complex (Drosophila melanogaster)
- The nematode worm C. elegans
Forsdyke, D. R. (2020) Biological Journal of the Linnean society (in press) Hybrid sterility can only be primary when acting as a reproductive barrier for sympatric speciation. arXiv Preprint Click Here
Forsdyke, D. R. (2019) Biological Journal of the Linnean society 127, (in press). Success of alignment-free oligonucleotide (kmer) analysis confirms relative importance of genomes not genes in speciation. (Click Here) arXiv Preprint Click Here
Forsdyke, D. R. (2018) arXiv Preprint Server. Confirmation that T cell receptor activation is digital, not analog. Click Here
Forsdyke, D. R. (2018) Encyclopedia of Life Sciences Mendel,Gregor Johann. Click Here [doi: 10.1002/9780470015902.a0002544.pub2]
Forsdyke, D. R. (2018) When few survive to tell the tale: thymus and gonad as auditioning organs.arXiv Preprint Server (Click Here)
Forsdyke, D. R. (2018) Shift in Nomenclature not Thesis: Innate Immune Memory, Pathogen Dose and Opsonins.arXiv Preprint Server (Click Here)
After activation by the plant lectin, concanavalin A (Con-A), T lymphocytes enter G1 phase and enlarge, subsequently entering S phase and dividing. A set of human putative G0/G1 switch regulatory genes ("G0S" genes) were identified in our laboratory. The corresponding mRNAs were found to be differentially expressed in human blood lymphocytes shortly after the addition of Con-A. It was anticipated that such mRNAs would correspond to proteins involved in orchestrating either entry into the G1 phase of the cell cycle, or the immediate defence response to a foreign pathogen. Some of the genes have been found to play a general role in signal transmission, e.g. in the central nervous system (Click here).
The 8 genes selected for detailed analysis were G0S2, G0S3, G0S7, G0S8, G0S9, G0S19, G0S24 and G0S30. G0S2 expression is very sensitive to inhibition by the immunosuppressant, cyclosporin-A, and by TNF-alpha. It specifically inhibits triglyceride lipase and the RNA quadruplex-binding protein, nucleolin, so countering the latter's promotion of proliferation (of importance in certain leukaemias; CML). G0S3 and G0S7 are members of the FOS oncogene family. G0S8 has been renamed RGS2 (regulator of G-protein signalling number 2), although it also reacts with adenyl cyclase, and also with the kinase domain of a protein implicated in Parkinson's Disease. G0S9 is "preB cell colony enhancing factor" (PBEF) and Nicotinamide phosphoribosyl transferase (NAMPT), a key to protein deacylations (e.g. facilitating sirtuin-mediated histone deacylations and secretion of TNF-alpha). G0S19, which we reported in 1985 as a differentially expressed mRNA, corresponds to the chemokine MIP1alpha (or CCL3), the receptor for which (CCR5) is a co-receptor for HIV-1. Resistance to AIDS is correlated with copy numbers of G0S19/CCL3 and related genes, or defects in CCR5. G0S24 by virtue of its binding to AU-rich elements, destabilises HIV-1 RNA and the mRNAs of TNF-alpha and tumor suppressors (LATS2), and may prove important for AIDS therapy (Click Here). G0S30, which we also reported in 1985, corresponds to the murine EGR1 and rat NGF1A genes. These encode a transcription factor that regulates TNF-alpha and cell senescence, is a "gatekeeper" of the p53 tumor suppressor pathway, and is implicated in the selection of auditioning T-lymphocytes in the thymus. Some of the human genes have acquired names originally applied to the rodent homologs.
|Human G0 Switch Gene||
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This page was initiated in 1998, and last updated 15 Aug 2019, by D. R. Forsdyke
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