The Chin-Sang Lab Reads

Papers Selected in 2012


 MIG-10 isoforms, varying only in their N-terminal motifs, can localize to specific subcellular domains, organize the actin cytoskeleton at these domains, and instruct distinct neurodevelopmental outcomes downstream from Netrin.  AMPK contributes to survival during L1 diapause in a manner distinct from that by which it controls dauer diapause,  and AMPK suppresses TORC1 activity to maintain germline quiescence.

 

 

The B-type cholinergic motor neurons transduce the proprioceptive signal. A sensorimotor feedback loop operating within a specific type of motor neuron both drives and organizes body movement. See also this preview: 

                    

 Worm Developmental Dynamics Database (http://so.qbic.riken.jp/wddd/), which stores a collection of quantitative information about cell division dynamics in early Caenorhabditis elegans embryos with single genes silenced by RNA-mediated interference. 

Two recent studies describe mechanisms by which sexually dimorphic responses to pheromones in the nematode worm Caenorhabditis elegans are driven by differences in the balance of neural circuits that control attraction and repulsion behaviors.

 At present, of the 20,377 protein-coding genes in this organism, 6764 genes with associated molecular lesions are either deletions or null mutations 

 Perspective and link to paper that shows PTEN can be transferred between cells.

  

Scott Emmons' perspective on two new papers on oxytocin/vasopressin  signaling that regulates complex behaviors in C. elegans.

 

E. coli noncoding RNAs can regulate gene expression and physiological conditions of C. elegans and indicate that noncoding RNAs might have interspecies ecological roles.

How the stable MT cytoskeleton of a mature neuron is converted into the dynamically growing MT cytoskeleton of a regrowing axon.

Remote controlled worms: one interneuron pair (AIY) was sufficient to force the animal to locate, turn towards and track virtual light gradients.

 

performed RNA-seq of individually staged and dissected linker cells, comparing transcriptomes from linker cells of third-stage (L3) larvae, fourth-stage (L4) larvae, and nhr-67-RNAi-treated  (needed for linker cell migration) L4 larvae.

The endosome-associated protein, SID-5, promotes the transport of RNAi silencing signals between cells.

 

 insulin/IGF-1 signaling selectively engages AKT-2/DAF-16 to promote DNA damage-induced germ cell apoptosis downstream of CEP-1 through the Ras pathway.

Model figure.

 

DAF-18 PTEN inhibits, RAS/MAPK signaling in the vulval precursor cells and the inhibitory activity is partially independent of its PIP3 lipid phosphatase activity. Model figure.

 

The Caenorhabditis elegans TransgeneOme provides a platform for large-scale, tag-based protein function exploration under endogenous regulatory control.

 

Genome-wide RNA-interference-feeding screen for suppressors of lin-35/Rb. Of 26 suppressors identified, 17 fell into three functional classes: (1) ribosome biogenesis genes, (2) mitochondrial prohibitins, and (3) chromatin regulators. See this genetics primer on the screen.

 

 

UNC-40/DCC, SAX-3/Robo, and VAB-1/Eph differentially regulate the abundance and subcellular localization of the WAVE/SCAR actin nucleation complex and its activator, Rac1/CED-10 for epidermal morphogenesis.

 

Evidence for phosphorylation inhibiting PTEN.  IGFBP-2 bound RPTPβ which led to its dimerization and inactivation. This enhanced PTEN tyrosine phosphorylation and inhibited PTEN activity.

 

The authors describe a phenomenon, RNA-induced epigenetic silencing (RNAe) , in which the C. elegans Piwi pathway can initiate a state of gene silencing that is extremely stable across generations.

 

The wiring diagram of the male tail form a neural network for mating.  See also this perspective:

.  Can this be done in humans? Watch this TED talk.

 

 

The Drosophila AF-6/afadin homolog Canoe (Cno) functionally interacts with the slit-Robo pathway.

 

See also:

Driver Mutations in Melanoma: Lessons Learned From Bench-to-Bedside Studies. Domain landscapes of somatic mutations in cancer.

 

Use the C. elegans OrthoList to find cancer genes in C. elegans:

 

 

 

Acoustic tweezers can manipulate C. elegans. See movie

Engineering to go beyond the constraints of the C. elegans genome to extend its lifespan.

 

Is ventral enclsoure the nematode phylotypic stage. Also see this preiview.

 

Rac GTPase activity is controlled by different GEFs in distinct axon guidance contexts, explaining how Rac GTPases can specifically control multiple cellular functions.

 

An photo-inducible cell ablation method.

 

 

The ATPase ASNA-1 is required for both growth and the L1 diapause. ykt-6, mrps-2, mrps-10 and mrpl-43 were identified genes that cause asna-1 like larval arrest.

 

 

In Zebrafish  hypoxia induces ephrinB2a activation and disrupts axon pathfinding. Interestingly magnesium sulfate (used to improve neurodevelopmental outcomes in preterm births) protects against pathfinding errors by preventing the upregulation of ephrinB2a.

 

A role of EphA:ephrinA attractive reverse signaling in motor axon guidance and in vivo evidence of in-parallel forward Eph and reverse ephrin signaling function in the same neuronal population

 

Review on the mechanisms regulating PTEN expression and function, including transcriptional regulation, post-transcriptional regulation by non-coding RNAs, post-translational modifications and protein–protein interactions.

 

PTEN is involved in the control of cell proliferation, migration, and survival of both cancer and tumor-associated endothelial cells. Ideally, combining PTEN-targeted therapy to classical chemotherapy should be a promising strategy.

 

This review is on cellular mechanisms underlying directional motility of the growth cone. The cellular ensemble of cytoskeleton, adhesion, and membrane orchestrates the directed movement of growth cones. 

UNC-6 (Netrin), is required for self-avoidance of sister dendrites from the PVD nociceptive neuron in Caenorhabditis elegans. UNC-40 receptor captures UNC-6 at the tips of growing dendrites for interaction with UNC-5 on the apposing branch to induce mutual repulsion.

 

New reagents that improve the efficiency, facilitate the selection for transgenic strains and expand the set of MosSCI insertion sites. See also:

 and this chapter.

 

 

The ability of PTEN to decrease neuronal spine density depended upon its protein phosphatase activity (not lipid) and its own phosphorylation status.

Short review on PTEN.

Super-PTEN mice have resistance to cancer and are less prone to obese due to increased energy expenditure and reduced body fat.

 

A summary of common genes that are regulators of neurodegenerative diseases obtained from model organism research.

 

While most genes when mutated don't lead to a gross observable phenotype, the Fraser lab demonstrate that, most agenes affect fitness, and this suggests that genetic networks are not robust to mutation.

 

RAE-1 binds RPM-1 and functions downstream of rpm-1 in neurons.

This is the first time that a key player of the PCP pathway is shown to be involved in the insulin/IGF-1-like signaling dependent modulation of life span in C. elegans.

 

VAPB/ALS8 major sperm protein domain (vMSP) are secreted by neurons act on Lar-like protein-tyrosine phosphatase (CLR-1) and Roundabout (SAX-3) growth cone guidance receptors expressed in striated muscle. This promotes striated muscle energy production and metabolism, in part through the regulation of mitochondrial localization and function.

 

 

A novel molecular "clutch" which anchors the preexisting contracting network of actomyosin in cells to contact points on neighbouring cells.

 

Selected by: Lois Mulligan. Ephrin A reverse signaling is mediated by Ret RTK.