The Chin-Sang Lab Reads

Papers selected January 2016-


SFA-1 the C. elegans homologue of SF1, also known as branchpoint binding protein, BBPis specifically required for lifespan extension by dietary restriction and by modulation of the TORC1 pathway components AMPK, RAGA-1 and RSKS-1/S6 kinase. Overexpression of SFA-1 is sufficient to extend lifespan. 


Use of daf-2  in modeling phenotypic consequences of candidate human pathogenic mutations.


Bidirectional signaling between EphrinB1 and EphB3b coordinates the movements of the hepatic endoderm and adjacent lateral plate mesoderm (LPM), resulting in asymmetric positioning of the zebrafish liver.  See also this Dev. Cell preview


These results support the existence of a conserved pathway for EphB trans-endocytosis that removes the physical tether between cells, thereby enabling cell repulsion.

Periodic A/T clusters (PATCs) can prevent transgenes from  stochastic silencing in germline.

ZIG-10 mediates an interaction between excitatory neurons and epidermis


A modular approach to synthesis of programmed polyproteams, through spontaneous isopeptide bond formation between peptide tags . The polyproteams are linked through irreversible amide bonds and so are stable over time (if protected from proteases) and allow easy analysis by SDS/PAGE.  Link to Addgene vectors

A improved version of proximity-dependent biotin identification (BioID)  method to detect protein-protein interactions in living cells. Link to Addgene vectors


Review on using C. elegans to study energy balance and feeding behaviour.

Linear DNAs with short homologies (∼35 bases) engage in a highly efficient gene conversion mechanism. There may be a size limitation as they got 0% edits a 2.4kb dsDNA.

β-amyloid may play a protective role in innate immunity and infectious or sterile inflammatory stimuli may drive amyloidosis

By duplicating the PVD neuron, Yip and Hieman show that dentrite tiling uses a mutual repulsion that rquires Netrin signaling.


Watch out Crispr/Cas9 there is a new kid on the block.  NgAgo a new gene editing too.  This protein uses a ssDNA guide (24nt with 5’Phosphate group) to make presice cuts in dsDNA.

Update:  There has been a lot of controversy about this method as many researcher are unable to reproduce the results. See Nature News


The Sherwood lab identified cdc-42 and gdi-1 are needed for invadopodia formation. CDC-42 is required for the invasive membrane localization of WSP-1 (N-WASP),  and GDI-1 mediates membrane trafficking.

sex-shared neurons adopt sex-specific synaptic wiring patterns. Reversal of the sexual identity of either the pre- or postsynaptic neuron alone transforms the patterns of synaptic connectivity to that of the opposite sex.  See Douglas Portman’s Nature News and Views article.

Review on Eph-ephrin signaling in the nervous system

A novel form of regeneration that is largely independent of defined regeneration pathways. This is similar to reduction of sensory activity which triggers axon regeneration in the mammalian CNS


3D graphic reconstruction of the developing intestine. LIN-12/Notch appears to modulate left-right adhesion in the primordium. Rotation appears to have a role in aligning the developing lumen of the intestine, and involves a conserved, UNC-6/netrin and other axon guidance genes.

Light-dependent activation of a guidance receptor, UNC-40/DCC using Arabidopsis thaliana Cryptochrome 2, which oligomerizes upon blue-light absorption. 

 

The GFP11 and sfCherry11 tags are only 18aa long and will reconstitute with FP1-10 to make a functional flourescent protein.  You can put these tags in tamdem to increase fluorescence. 

The Stome lab has shown that daf-2  long lived animals do not aquire a "germ cell program"  in the soma.  In contrast to this 2009 paper.

Optogenetics approach to control organelle transport.

 VAV-1, GEF activity acts in ALA to suppress locomotion and feeding during sleep-like behavioral quiescence in response to cellular stress. Additionally, VAV-1 activity is required for EGF-induced sleep-like quiescence and normal levels of EGFR and secretory dense core vesicles in ALA. 

vps-50 affects the behavioral state of C. elegans. VPS-50 and its murine homolog mVPS50 associate with synaptic and dense-core vesicles and function in acidification andmaturation of dense-core vesicles.


The authors extend the applicability of whole-genome sequencing to a broader spectrum of mutations, including classes that are difficult to map by traditional means.

EFN-4 has a new receptor.   L1 cell adhesion molecule LAD-2 acts as anephrin receptor to EFN-4 to promote axon guidance.  EFN-4 can function as a diffusible factor.


This paper supports our work we published in 2007 that EphR inhibits PTEN and in particular EphA7 overexpression role in promoting NSCLC.  Thus EphA7 may provide a targeted therapy for NSCLC.

Loss of the H3K4me3/2 histone emethylase, RBR-2 causes axon guidance defects due to the transcriptional up regulation of wsp-1.  The  NURF complex is required for the upreguation of wsp-1


PTEN deletion can rescue someof FGFR mutant phenotypes.

Drosphila insulin like peptide 8 works through the G protien coupled  Lgr3 relaxin receptor.


 

A new way to mutagenize worms using light and Histone-MiniSOG.


Trojanowski and Raizen Review on C. elegans sleep.