BIOL 205

BIOL 205 will be hosted through OnQ.

BIOL 205

Mendelian and Molecular Genetics

Fall Term (2016-17)

CALENDAR DESCRIPTION

An introduction to Mendelian and Molecular Genetics covering the basic mechanisms of genetic transmission, gene structure and function, as well as the application of molecular genetics in medicine and biotechnology. 

PREREQUISITE A GPA of 1.90 in BIOL 102/3.0 and BIOL 103/3.0.


SCHEDULE


Section 001:  Tu 14:30- 16: 00 Fr 16:00- 17:30

Section 002:   Tu 16:00-17:30  Th 14:30- 16:00


Instructor Dr. I. Chin-Sang 

Instructor Contact (chinsang@queensu.ca – Phone 613-533-6124) 

Lab Coordinator: 

Dr. Fern Gauthier , Rm. 3321 Biosciences complex, 533-6000 ext. 77666

e-mail: gauthier"at"queensu.ca

Office Hours E-mail instructor for appointments

TA: See Course Website

TA Contact Information See Course Website

Office Hours See TA in assigned  lab section 

Textbook: 

Introduction to genetic analysis 11th edition by Anthony Griffiths et al. WH Freeman. Purchase at campus bookstore . At least 2 copies of the textbook will be on reserved (3 hour loan) in the Stauffer Library.  

Learning Objectives

The goals of Biology 205 is to make genetics understandable, demonstrate its relevance, and to improve students’ critical thinking skills. I don’t want students just memorizing concepts without understanding how experiments are done and why they are done that way. This is probably not your first exposure to genetics analysis, but there will be new concepts you haven't learned so I will provide the necessary background such that you can understand these concepts. The second half of the course will focus on the molecular mechanisms that explain genetic principles. Students are expected to read the assigned readings and are encouraged to ask questions in lectures. 

Learning Hours

Teaching method Average hours per week, Number of weeks, Total hours

In-class hours Lecture 3, 12, 36

Seminar NA

Laboratory 3, 6, 18

Tutorial 3, 6. 18

Practicum NA

Group learning NA

Individual instruction  NA

Other Online activity 1, 12, 12

Off-campus activity NA

Private study 3, 12, 36

Total hours on task 120

Course Outline

Please see course website for details

Genetics and the questions of biology 

The molecular basis of genetic information

The program of genetic investigation

Methodologies used in genetics

Model organisms

Genes, the environment, and the organism 

Genes and chromosomes 

Single-gene inheritance patterns

The chromosomal basis of single-gene inheritance patterns

Discovering genes by observing segregation ratios

Sex-linked single-gene inheritance patterns, Human pedigree analysis 

Mendel’s law of independent assortment 

Working with independent assortment

The chromosomal basis of independent assortment

Polygenic inheritance

Organelle genes: inheritance independent of the nucleus 

Diagnostics of linkage 

Mapping by recombinant frequency

Mapping with molecular markers

Centromere mapping with linear tetrads

Using the chi-square test for testing linkage analysis

Accounting for unseen multiple crossovers

Using recombination-based maps in conjunction with physical maps 

Working with microorganisms 

Bacterial conjugation

Bacterial transformation

Bacteriophage genetics, transduction

Physical maps and linkage maps compared 

Interactions between genes 

Interactions between the alleles of a single gene: variations on dominance

Interaction of genes in pathways, Inferring gene interactions

Penetrance and expressivity

Introduction to molecular genetics 

Molecular developmental genetics

Why are emerging (bio)technologies often controversial? 

DNA 

Structure, replication

Mutation, repair and recombination 

Induced mutations, repair and cancer, chromosomal aberrations 

RNA

Transcription and Processing

RNA polymerase and transcription factor assemblages

Splicing, and non coding RNAs 

Protein

Protein and their synthesis, tRNA, Ribosomes

The proteome

Regulation of Gene Expression

Gene regulation in Bacteria, viruses, and Eukaryotes

Epigenetics, Chromatin remodelling and DNA methylation

Recombinant DNA 

Gene isolation and Manipulation, restriction enzymes, plasmids, restriction enzyme independent cloning, next generation sequencing, RNA Seq., 

Genome Editing with Crispr Cas9, 

Techniques and applications

Genomes and Genomics

The Genomics revolution

Bioinformatics

Comparative genomics and Human medicine

Textbooks/Readings

See course website

Grading Scheme

Component Weight (%) Date

Midterm exam 25 See course website

Quizzes/Clickers 10 See course website

Lab Report/Assignments 30 See course website

Lab Quizzes 10 See course website

Final exam 25 See course website

Grading Method

In this course, some components will be graded using numerical percentage marks.  Other components will receive letter grades, which for purposes of calculating your course average will be translated into numerical equivalents using the Faculty of Arts and Science Letter Grade Input Scheme.

When letter grades are employed, the following scale will be employed for purposes of calculating your course average:

Arts & Science Letter Grade Input Scheme

Assignment mark Numerical value for calculation of final mark

A+ 93

A 87

A- 82

B+ 78

B 75

B- 72

C+ 68

C 65

C- 62

D+ 58

D 55

D- 52

F48 (F+) 48

F24 (F) 24

F0 (0) 0

Your course average will then be converted to a final letter grade according to Queen’s Official Grade Conversion Scale:

Queen’s Official Grade Conversion Scale

Grade Numerical Course Average (Range)

A+ 90-100

A 85-89

A- 80-84

B+ 77-79

B 73-76

B- 70-72

C+ 67-69

C 63-66

C- 60-62

D+ 57-59

D 53-56

D- 50-52

F 49 and below

Academic Integrity and Queen’s Code of Conduct

Students are responsible for familiarizing themselves with the regulations concerning academic integrity and for ensuring that their assignments and conduct conform to the principles of academic integrity. Information is available in the Arts and Science Calendar (see Academic Regulation 1 -  http://www.queensu.ca/artsci/academic-calendars/regulations/academic-regulations, on the Arts and Science website (see http://www.queensu.ca/artsci/academics/undergraduate/academic-integrity), and at Biology’s website (http://www.queensu.ca/biology/undergrad/integrity.html) and from the instructor of this course. Departures from academic integrity include plagiarism, use of unauthorized materials, facilitation, forgery and falsification, and are antithetical to the development of an academic community at Queen's. Given the seriousness of these matters, actions which contravene the regulations on academic integrity carry sanctions that can range from a warning or the loss of grades on an assignment to the failure of a course to a requirement to withdraw from the university.

Accommodation Policy, Exam Conflicts, and Other Conflicts

Students who feel they need accommodations for disabilities or extenuating circumstances, or have a conflict between exams or other commitments should consult the Biology Department’s website for details about how to proceed (http://www.queensu.ca/biology/undergrad/integrity.html). In general, the earlier a course coordinator is apprised of an extenuating circumstance, the more likely an accommodation can be made. Students are encouraged to be proactive in anticipating difficulties, when it is possible to do so.

Students may apply to write a make-up or deferred exam if they have an exam conflict as defined in the Academic Regulations of the Faculty (See Arts and Science Calendar Regulation 8 - http://www.queensu.ca/artsci/academic-calendars/regulations/academic-regulations). In this case, the student should report to the Exams Office first to verify that there is a genuine exam conflict. Biology professors will not consider your situation to be a conflict unless it meets the criteria set out by the Faculty of Arts and Sciences.

Students may request a make-up or deferred exam if they have an exam conflict with off-campus travel associated with a field course (e.g BIOL-307/3.0 or 407/3.0) that is held during the fall or winter terms.

Copyright

 This material is designed for use as part of BIOL 205 at Queen’s University and is the property of the instructor unless otherwise stated. Third party copyrighted materials (such as book chapters and articles) have either been licensed for use in this course or fall under an exception or limitation in Canadian Copyright law.

Accommodation of Disabilities

Queen's University is committed to achieving full accessibility for persons with disabilities. Part of this commitment includes arranging academic accommodations for students with disabilities to ensure they have an equitable opportunity to participate in all of their academic activities. If you are a student with a disability and think you may need accommodations, you are strongly encouraged to contact the Disability Services Office (DSO) and register as early as possible. For more information, including important deadlines, please visit the DSO website at: http://www.queensu.ca/hcds/ds/