Rapid Ligation Buffer

2X Rapid Ligation Buffer

 

Recipe from NEB:

2X Quick Ligation Buffer:
132 mM Tris-HCl (pH= 7.6 @ 25°C), 20 mM MgCl2, 2 mM dithiothreitol, 2 mM ATP, 15% Polyethylene glycol (PEG 6000)*

Recipe from Promega:

2X Rapid Ligation Buffer

60mM Tris-HCl (pH 7.8)

20mM MgCl2

20mM DTT

2mM ATP

10% polyethylene glycol