Homemade Colloidal Coomassie Blue Staining

 Working Solution (1L):

In In a large beaker add the ingredient in the order listed.  Make sure the Ammonium Sulfate is dissolved before adding the Coomassie G-250 solution to a final of 0.1%. Bring final volume up to 1L with H20. Store at room temperature with a tightly sealed lid.


 1.  After SDS PAGE rinse Gel twice with 100 ml Distilled H20 (3 minutes each wash).

2.  Add Coomassie Blue working solution to Gel and stain for at least 2 hours.

3.  Bands should be visible without de-staining but you can de-stain with H20.


This Protocol was modified from references:

 Kang et al. (2002).  Highly Sensitive and Fast Protein Detection with Coomassie Brilliant Blue in Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis Bull. Korean Chem. Soc. 2002, vol. 23, No. 11 1511-1512.

 Neuhoff et al. (1988). Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250.Electrophoresis. 1988 Jun;9(6):255-62.



M    Induced   Uninduced     Purified