5X Long PCR Buffer

 

This buffer seems to work well with Worm Genomic DNA using Taq/Pfu (3:1) mixture.

 

5X Long PCR Buffer (store in 1ml aliquots in -20C)

  • 425 mM KOAc
  • 125 mM Tricine pH 8.7 (adjust pH of Tricine stock solution with KOH)
  • 40% glycerol
  • 5% DMSO
  • 1.2 mM Mg(OAc)2

10 ml 5X buffer

  • 4.25 ml 1M KOAc
  • 1.25 ml 1M Tricine, pH8.7 @ 25 degrees C (with KOH)
  • 4.00 ml glycerol
  • 0.50 ml DMSO
  • 120 ul 500 mM Mg(OAc)2