1. I Brockhausen, E Hull, O Hindsgaul, H Schachter, R Shah, S Michnick, JP Carver. Control of Glycoprotein Synthesis. Detection and characterization of a novel branching enzyme from hen oviduct, UDP-N-Acetylglucosamine: GlcNAc β1,4 (GlcNAc β1-2) Man-R (GlcNAc to Man) β1,4-N-acetylglucosaminyltransferase VI-(GlcNAcβ1,2) Man-R(GlcNAc to Man). (1989) J Biol Chem 264:11211-11221.
2. I Brockhausen, W Kuhns, H Schachter, KL Matta, R Sutherland, MA Baker. Biosynthesis of O-glycans in leukocytes from normal donors and from patients with leukemia: increase in O-glycan core 2 UDP-GlcNAc: Gal β3GalNAc-R (GlcNAc to GalNAc) β1,6-N-acetylglucosaminyltransferase in leukemic cells. (1991) Cancer Res 51:1257-1263.
3. W Kuhns, V Rutz, H Paulsen, KL Matta, MA Baker, M Barner, M Granovsky,
I Brockhausen. Processing O-glycan core 1, Galβ1-3GalNAc-R
(GlcNAc to GalNAc) by
core 2, UDP-GlcNAc:Galβ1-3GalNAc-R
β6-N-acetylglucosaminyltransferase and CMP-sialic acid: Gal 1-Alpha 3-sialyltransferase. (1993) Glycoconj J10:381-394.
4. A Pollex-Krüger, B Meyer, R Stuike-Prill, V Sinnwell, KL Matta, I Brockhausen. Preferred Conformations and Dynamics of five core structures of mucin-type O-glycans determined by NMR spectroscopy and force field calculations. (1993) Glycoconj J 10:365-380.
5. M Granovsky, T Bielfeldt, S Peters, H Paulsen, M Meldal, J Brockhausen, I Brockhausen. UDP-galactose: glycoprotein-N-acetyl-D-galactosamine 3-β-D-galactosyltransferase activity synthesizing O-glycan core 1 is controlled by the amino acid sequence and glycosylation of glycopeptide substrates. (1994) Eur J Biochem 221: 1039-1046.
6. F Vavasseur, K Dole, J. Yang, K. L. Matta, N. Myerscough, A Corfield, C Paraskeva, I Brockhausen. O-glycan biosynthesis in human colorectal adenoma cells during progression to cancer. (1994) Eur J Biochem 222: 415-424.
7. J Yang, J Byrd, B Siddiki, YS Chung, M Okuno, M Sowa, YS Kim, KL Matta,
I Brockhausen. Alterations of O-glycan biosynthesis in human colon cancer
tissues. (1994) Glycobiology 4: 873-884.
8. F Vavasseur, J Yang, K Dole, H. Paulsen, I Brockhausen. Synthesis of O-glycan core 3: Characterization of UDP-GlcNAc: GalNAc-R β3-N-acetyl-glucosaminyltransferase activity from colonic mucosal tissues and lack of the activity in human cancer cell lines. (1995) Glycobiology 5: 351-357.
9. W Kuhns, R Jain, KL Matta, H Paulsen, MA Baker, R Geyer, I Brockhausen. Characterization of a novel sulphotransferase activity synthesizing sulphated O-glycan core 1, 3-sulphate-Galβ1-3GalNAcα-R. (1995) Glycobiology 5: 689-697.
10. I Brockhausen, J Yang, J Burchell, C Whitehouse, J Taylor-Papadimitriou. Mechanisms underlying aberrant glycosylation of MUC1 mucin in breast cancer cells. (1995) Eur J Biochem 233: 607-617.
11. I Brockhausen, J Yang, N Dickinson, S Ogata, S Itzkowitz. Enzymatic basis for sialyl- Tn expression in human colon cancer cells. (1998) Glycoconj J15: 595-603.
12. I Brockhausen. J Schutzbach, W Kuhns. Glycoproteins and their relationship to human disease. (1998) Acta Anat. 161: 36-78.
13. I Brockhausen. Pathways of O-glycan biosynthesis in cancer cells. (1999) BBA. 1473: 67-95.
14. M Dalziel, C Whitehouse, I McFarlane, I Brockhausen, S Gschmeissner, T Schwientek, H Clausen, J Burchell, J Taylor-Papadimitriou. The relative activities of the C2GnT1 and ST3Gal- I glycosyltransferases determine O-glycan structure and expression of a tumor-associated epitope on MUC1. (2001) J Biol Chem 276: 11007-11015.
15. I Brockhausen, F Vavasseur, X. Yang. Biosynthesis of mucin type O-glycans: lack of correlation between glycosyltransferase and sulfotransferase activities and CFTR expression. (2001) Glycoconjugate J 18: 685-697.
16. I Brockhausen, M Lehotay, JM Yang, W Qin, D Young, J Lucien, J Coles, H Paulsen. Glycoprotein biosynthesis in porcine aortic endothelial cells and changes in the apoptotic cell population. (2002) Glycobiology, 12: 33-45.
17. X Yang, W Qin, M Lehotay, D Toki, P Dennis, JS Schutzbach, I Brockhausen. Soluble human core 2 β6-N-acetylglucosaminyltransferase C2GnT1 requires its conserved cysteine residues for full activity (2003) BBA 1648:62-74.
18. X Yang, M Lehotay, T Anastassiades, M Harrison, I Brockhausen. The effect of TNFalpha on glycosylation pathways in bovine synoviocytes. (2004) Biochemistry and Cell Biology 82(5):559-568.
19. A Mungul, L Cooper, I Brockhausen, K Ryder, U Mandel, H Clausen, A Rughetti, DW Miles, J Taylor-Papadimitriou, JM Burchell. Sialylated core 1 based O-linked glycans enhance the growth rate of mammary carcinoma cells in MUC1 transgenic mice. (2004) Internat J Oncol, 25(4):937-943.
20. DS McBride, I Brockhausen. FWK Kan. Detection of glycosyltransferases in the golden hamster (Mesocricetus auratus) oviduct and evidence for the regulation of O-glycan biosynthesis during the estrus cycle. (2005) Biochim Biophys Acta.1721:107-115.
21. JG. Riley, M Menggad, P Montoya-Peleaz, WA. Szarek, CL Marolda, MA Valvano, JS Schutzbach, I Brockhausen. The wbbD gene of E. coli strain VW187 (O7:K1) encodes a UDP-Gal: GlcNAc(alpha)-pyrophosphate-R (beta)1,3-galactosyltransferase involved in the biosynthesis of O7-specific lipopolysaccharide. (2005) Glycobiology 15: 605-613.
22. I Brockhausen, M Benn, S Bhat, S Marone, JG Riley J, P Montoya-Peleaz, JZ Vlahakis, H Paulsen, JS Schutzback, W Szarek. UDP-Gal: GlcNAc-R Beta1,4-Galactosyltransferase - a target enzyme for drug design. Acceptor specificity and inhibition of the enzyme. (2006) Glycoconj J
23. I Brockhausen. Mucin-type O-glycans in human colon and breast cancer: glycodynamics and functions. (2006) EMBO Reports 7: 599-604
24. I Brockhausen. The role of galactosyltransferases in cell surface functions and in the immune system. (2006) Drug News and Perspectives 19(7): 401-409.
25. X Yang, J Yip, T Anastassiades, M Harrison, I Brockhausen. The action of TNFalpha and TGFbeta include specific alterations of the glycosylation of bovine and human chondrocytes. (2007) BBA, 1773 (2): 264-272.
26. J Schutzback, H Ankel, I Brockhausen. Synthesis of cell envelope glycoproteins of Cryptococcus laurentii. (2007) Carboyhdr. Res. 342: 881-893.
27. Y Li, X Yang, A Nguyen, I Brockhausen, Requirement of N-glycosylation for the secretion of recombinant extracellular domain of human Fas in HeLa cells. (2007) Internat. J biochem Cell Biol 39: 1625-1636.
28. X Yang, J Yip, M Harrison, I Brockhausen. Primary human osteoblasts and bone cancer cells as models to study glycodynamics in bone. (2008) Internat. J biochem Cell Biol 40(3): 471-483.
29. I Brockhausen, J Riley, M Joynt, XYang, WA. Szarek. Acceptor substrate specificty of UDP-Gal: GlcNAc-R β1,3-galactosyltransferase from Escherichia coli O7:K1, Glycoconj J 25: 663-673, 2008.
30. I Brockhausen, B Liu, B Hu, K Lau, W Szarek, L Wang, L Feng. Characterization of two UDP-GlcNAc: β1,3-glucosyltransferases from the Escherichia coli serotypes O56 and O152. J Bacteriol 190: 4922-4932, 2008.
31. I Brockhausen, T Dowler, H Paulsen. Site directed processing: Role of amino acid sequences and glycosylation of acceptor glycopeptides in the assembly of extended mucin type O-glycan core 2. Biochimica et Biophysica Acta, 1790: 1244-1257, 2009.
32. J Riley, C Xu, I Brockhausen. Synthesis of acceptor substrate analogs for the study of glycosyltransferases involved in the second step of the biosynthesis of O antigen repeating units. Carbohydr Res 345:586-597, 2010.
33. G Picco, S Julien, I Brockhausen, R Beatson, A Antonopoulos, S Haslam, U Mandel, A Dell, S Pinder, J Taylor-Papadimitriou and J Burchell. Over-expression of ST3Gal-I promotes mammary tumorigenesis. Glycobiology 20:1241-1250, 2010.
34. Y Gao, Lazar C, WA. Szarek, I Brockhausen. Specificity of b4galactosyltransferase inhibitor 2-naphthyl 2-butanamido-2-deoxy-1-thio-b-D-glucopyranoside. Glycoconj J 27(7-9):673-684, 2010.
35. C Xu, B Liu, B Hu, Y Han, L Feng, J Allingham, WA Szarek, L Wang, I Brockhausen. Biochemical characterization of a new enzyme from Shigella boydii type 14 that catalyzes the second step in O-antigen repeating unit synthesis: UDP-Gal:GlcNAc-pyrophosphate lipid b1,4-galactosyltransferase WfeD. J Bacteriol 193(2):449-459, 2011.
Books and selected book chapters:
1. I Brockhausen, W Kuhns. Glycoproteins and human disease. Medical Intelligence Unit, CRC Press and Mosby-Year Book, Chapman & Hall NY (1997) Publisher: R.G.Landes Company, 810 S. Church Street, Georgetown Texas USA 78626. Distributor: Springer-Verlag, Heidelberg, Germany
2. I Brockhausen, editor: Glycobiology Protocols. Methods in Molecular Biology (2006) Publisher: Humana Press.
3. I Brockhausen, H Schachter, P Stanley. ‘O-glycans’ (2007) In Essentials of Glycobiology. 2nd. edition. Edited by Varki, A., Cummings, R. D., Esko, J.D., Freeze, H.H., Stanley, P., Bertozzi, C.R., Hart, G.W., and Etzler M.E. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 2008.
4. I Brockhausen. Biochemical Aspects: Biosynthesis of mucin-type O-glycans. (2007) In Comprehensive Glycosciences 'From Chemistry to Systems Biology'. JP Kamerling ed. Acad. Press/Elsevier, Burlington MA. pp33-59.
5. I Brockhausen. Glycosyltransferases specific for the synthesis of mucin-type O-glycans. (2006). In 'Glycobiology', C Sansom, O Markman eds. Scion Publ Ltd, Woodbury NY. pp217- 234.
6. I Brockhausen, T Anastassiades. Relationship between inflammation and glycosylation in arthritis. (2008) Expert Review of Clinical Immunology 4(2): 173-191.
7. J Schutzbach, I Brockhausen. Inhibition of glycosyltransferase activities as the basis for drug development. (2009) Methods in Molecular Biology, Humana Press, Totowa NY. 534:1-15.
8. I Brockhausen. Biosynthesis of complex mucin-type O-glycans. (2009)
Comprehensive Natural Products Chemistry II Volume 5, Carbohydrates,
nucleosides and nucleic acids. G. Wang Ed. Elsevier.
Inka Brockhausen is an associate professor (adjunct 1) in the Departments
of Medicine and Biochemistry at Queen’s University, Kingston, Ontario. Her
research interest includes the glycobiology of arthritis and inflammatory
diseases, cystic fibrosis, cytokine actions, control of cell death, and the development of
anti-inflammatory drugs. The Brockhausen laboratory enjoys many expert
collaborators that play an essential role in the progress of the research projects. Based on biochemical characterization of the enzymes involved in
the biosynthesis of glycoproteins, the team has discovered several
carbohydrate-based enzyme inhibitors that could potentially block
inflammation, as well as the spread of cancer cells and reduce the pathology
of lung disease involving highly viscous mucus and adhesive carbohydrates. A
very successful area of research is the study of the enzymatic synthesis of
bacterial polysaccharides and the development of O antigen synthesis
inhibitors which could be applied as new anti-bacterial agents or block the
attachment of bacteria to mucosa. Previous work by Dr. Brockhausen at the Hospital for Sick
Children and the University of Toronto included the discovery of new pathways
and abnormal glycoprotein biosynthesis in colon and breast cancer, leukemia
and cystic fibrosis. Dr. Brockhausen received several prestigious awards,
including a Scientist award from the Medical Research Council and a Research
Scientist award from the Arthritis Society, as well as financial support from
the Medical Research Council, CIHR, NSERC, Materials and Manufacturing
Ontario, Cystic Fibrosis Foundation, Heart & Stroke Foundation of Canada,
Mizutani Glycosciences Prostate Cancer Fight Foundation and Queen’s University.